Personalities

Stanislav A. Vasilyev

Stanislav A. Vasilyev, PhD, senior researcher of Laboratory of Cytogenetics of Research Institute of Medical Genetics of Tomsk NRMC.

Contact details

e-mail: stanislav.vasilyev@medgenetics.ru
Phone: 8 (3822) 51-31-46
fax: 8 (3822) 51-37-44

Brief Biography

S. Vasilyev was born in 1984, he graduated with honors in 2007 from the Master of Biology and Soil Faculty of Tomsk State University. From 2007 to 2010 he was a PhD student in Research Institute of Medical Genetics. From 2010, he was a research assistant at the cytogenetics laboratory, and from 2014, he was a senior researcher. In 2010 he defended his PhD thesis in the specialty "genetics".

Research Interests

The main area of interest is the origin mechanisms of numerical and structural chromosomal abnormalities in human somatic cells, depending on the effects of external and internal factors.
In 2010, he defended his PhD thesis on the effect of low doses of incorporated plutonium-239 on the frequency of aneuploidy in human somatic cells. As a main result the effect of plutonium-239 in peripheral blood lymphocytes of radiochemical production workers on the frequency of chromosomal non-disjunction and loss was determined for the first time. In addition, an analysis was made of the contribution of these mechanisms to the overall frequency of aneuploidy in human somatic cells (Timoshevsky et al., 2010).
The results of the PhD thesis allowed to characterize the aneugenic effect of radiation as a new component in the assessment of genotoxic risks (Lebedev et al., 2011) and were presented in the monograph (Ilinskih et al., 2011). In continuation his studies, the induction of aneuploidy after exposure to ionizing radiation was also shown in the cells of cancer patients during neutron therapy (Melnikov et al., 2012; Melnikov et al., 2012).
The main idea of the subsequent studies is the hypothesis about the effect of the epigenetic background on spontaneous and induced mutagenesis in human somatic cells during ontogenesis. Within these researches, the methylation level of the retrotransposon LINE-1, constituting about 20% of the human genome, and the spontaneous level of γH2AX foci, which are the main marker of double-stranded DNA breaks in cells, are considered as important components of the epigenetic background. The effect of the spontaneous level of γH2AX foci on the origin of chromosomal abnormalities and cell viability was detected in hematopoietic stem cells (Vasilyev et al., 2013), tumor cell lines (Markova et al., 2015), peripheral blood lymphocytes of healthy individuals (Vasilyev et al., 2012; 2015) and cancer patients (Markova et al., 2015). Currently, a wide-transcriptome analysis has been carried out in human lymphocytes and genes have been identified, the expression of which is associated with both the spontaneous level of γH2AX foci and the frequency of the resulting chromosomal abnormalities after mutagenic exposure. It was shown, that the spontaneous level of γH2AX foci is an important component of the epigenetic background, which determines the sensitivity of cells and the appearance of structural chromosomal abnormalities after mutagens activity.
The level of methylation of LINE-1 can potentially affect the expression and activity of proteins of this retrotransposon, as well as activate insertional mutagenesis, leading to structural chromosomal abnormalities. In addition, a change in the level of LINE-1 methylation in cells is potentially associated with the conformation of the centromere regions of chromosomes and may lead to the generation of aneuploidy. Therefore, a violation of the LINE-1 methylation level in individual somatic cells may be associated with an increase in the frequency of numerical and structural chromosomal abnormalities, and, if it occurs in embryogenesis, lead to the death of the whole organism. Indeed, we found a reduced level of LINE-1 methylation in the placental tissues of spontaneous abortions of the first trimester of pregnancy with a normal karyotype compared with normally developing embryos (Vasilyev et al. 2015). LINE-1 hypermethylation can also potentially be associated with abnormal intrauterine development, but by itself does not seem to lead to abortion, since it does not occur among spontaneous abortions with a normal karyotype (Vasilyev et al., 2015). However, LINE-1 hypermethylation occurs in the chorionic trophoblast on the background of karyotype abnormalities. Thus, an elevated level of methylation of LINE-1 was found in the chorionic trophoblast of spontaneous abortuses with mosaic forms of aneuploidy, in contrast to pure forms (Vasilyev et al., 2015). In addition, in the lymphocytes of the peripheral blood of adult individuals, a link was found between the hypomethylation of LINE-1 and an increase in the frequency of chromosomal loss.

Achievements, awards, and grants

The results of S. Vasilyev over the past five years has been reflected in numerous publications, among which 25 articles in peer-reviewed national and foreign journals.

For the period 2015-2020 S. Vasilyev was the head of four and the performer of five grants from various scientific foundations - the Russian Science Foundation, Russian Foundation for Basic Research, Grants and scholarships of the President of Russian Federation. Currently S. Vasilyev is a member of the Vavilov Society of Genetics and Breeders, Russian Society of Medical Geneticists and the European Society for Radiation Research.

S. Vasilyev was awarded the Certificate of acknowledgement by the Administration of Tomsk region (2011), Award in education, science, health care and culture of Tomsk region (2016), Certificate of honour of Administration of Tomsk city (2017), Certificate of honour of Administration of Tomsk region (2018), Certificate of Acknowledgement by the Health defense committee of State Duma of the Russian Federation (2018), Memorial sign "The Future of the Tomsk region" (2019).

Main Publications

1. Vasilyev S.A., Tolmacheva E.N., Kashevarova A.A., Sazhenova E.A., Lebedev I.N. Methylation status of LINE-1 retrotransposon in chromosomal mosaicism during early stages of human embryonic development // Molecular Biology, 2015, Vol. 49, No. 1, pp. 144–152. http://link.springer.com/article/10.1134%2FS0026893314060193


2. Markova E., Vasilyev S., Belyaev I. 53BP1 foci as a marker of tumor cell radiosensitivity // Neoplasma. 2015. 62(5):770-6. doi: 10.4149/neo_2015_092. http://www.ncbi.nlm.nih.gov/pubmed/26278144


3. Vasilyev S.A., Velichevskaya A.I., Vishnevskaya T.V., Belenko A.A., Gribova O.V., Plaksin M.B., Startseva Zh.A., Lebedev I.N. Background Level of gammaH2AX Foci in Human Cells as a Factor of Individual Radiosensitivity // Radiatsionnaia biologiia, radioecologiia. 2015. Vol. 55. № 4. P. 402. http://elibrary.ru/item.asp?id=23908902


4. Denisov E.V., Skryabin N.A., Vasilyev S.A., Gerashchenko T.S., Lebedev I.N., Zavyalova M.V., Cherdyntseva N.V., Perelmuter V.M. Relationship between morphological and cytogenetic heterogeneity in invasive micropapillary carcinoma of the breast: a report of one case // Journal of Clinical Pathology. 2015. doi: 10.1136/jclinpath-2015-203009. http://jcp.bmj.com/content/early/2015/06/15/jclinpath-2015-203009


5. Marková E., Somsedíková A., Vasilyev S., Pobijaková M., Lacková A., Lukačko P., Belyaev I. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy // International journal of radiation biology. 2015. Vol.91, №12. P. 934-945. doi:10.3109/09553002.2015.1101498 http://www.tandfonline.com/doi/abs/10.3109/09553002.2015.1101498?journalCode=irab20


6. Tolmacheva E.N., Vasilyev S.A., Sazhenova E.A., Zhigalina D.I., Grigorovich E.I., Nikitina T.V., Melnikov A.A., Zhabina E.S., Ivanova T.V., Evtushenko I.D., Lebedev I.N. Skewed X-chromosome inactivation in human miscarriages // Cell and Tissue Biology. 2016. Vol. 10, № 1. P. 55-59. doi:10.1134/S1990519X16010119 http://link.springer.com/article/10.1134/S1990519X16010119


7. Nikitina T.V., Sazhenova E.A., Tolmacheva E.N., Sukhanova N.N., Kashevarova A.A., Skryabin N.A., Vasilyev S.A., Nemtseva T.N., Yuriev S. Yu., Lebedev I.N. Comparative cytogenetic analysis of spontaneous abortions in recurrent and sporadic pregnancy losses // Biomedicine Hub. 2016. Vol. 1, No. 1. 446099. doi:10.1159/000446099 https://www.karger.com/Article/FullText/446099


8. Vasilyev S.A., Urazova A.S., Nikitina T.V., Sazhenova E.A., Tolmacheva E.N., Agab A.V., Zhigalina D.I., Lebedev I.N. Loss of chromosome fragments or whole chromosomes in fibroblasts of extraembryonic mesoderm of spontaneous abortions with aneuploidy and triploidy // Meditsinskaya genetika. 2016. Vol. 15, № 4. P. 14-16. http://www.medgen-journal.ru%2Fjour%2Farticle%2Fdownload%2F109%2F97&usg=AFQjCNF-3iwRqt9msKqizvZ2zMOwdPqMfA&sig2=lBiojHxEeH8nRTUvGanA9w&cad=rja


9. Belenko A.A., Vasilyev S.A., Lebedev I.N. Markers of the individual radiosensitivity of human extraembryonic cells in vitro // Russian Journal of Genetics: Applied Research. 2017. Vol. 7, № 2. P. 203-204. http://link.springer.com/article/10.1134/S2079059717020034


10. Vasilyev S.A., Tolmacheva E.N., Lebedev I.N. Epigenetic Regulation and Role of LINE-1 Retrotransposon in Embryogenesis // Russian Journal of Genetics Volume. 2016. Vol. 52, № 12. P. 1219-1226. DOI: 10.1134/S1022795416120152 http://link.springer.com/article/10.1134/S1022795416120152


11. Skryabin, N.A., Vasilyev, S.A., Lebedev, I.N. Epigenetic silencing of genomic structural variations // Russian Journal of Genetics. 2017. Vol. 53. P. 1072-1079. DOI: 10.1134/S1022795417100106 https://link.springer.com/article/10.1134%2FS1022795417100106


12. Kashevarova A.A., Belyaeva E.O., Nikonov A.M., Plotnikova O.V., Skryabin N.A., Nikitina T.V., Vasilyev S.A., Yakovleva Yu.S., Babushkina N.P., Tolmacheva E.N., Lopatkina M.E., Savchenko R.R., Nazarenko L.P., Lebedev I.N. Compound Phenotype in a Girl with r(22), Concomitant Microdeletion 22q13.32-q13.33 and Mosaic Monosomy 22 // Molecular Cytogenetics. 2018. Vol. 11, № 26. doi: 10.1186/s13039-018-0375-3.


13. Kashevarova A.A., Nazarenko L.P., Skryabin N.A., Nikitina T.V., Vasilyev S.A., Tolmacheva E.N., Lopatkina M.E., Salyukova O.A., Chechetkina N.N., Vorotelyak E.A., Kalabusheva E.P., Fishman V.S., Kzhyshkowska J., Graziano C., Magini P., Romeo G., Lebedev I.N. A mosaic intragenic microduplication of LAMA1 and a constitutional 18p11.32 microduplication in a patient with keratosis pilaris and intellectual disability // Am J Med Genet A. 2018 Sep 23. doi: 10.1002/ajmg.a.40478.


14. Nikitina T.V., Menzorov A.G., Kashevarova A.A., Gridina M.M., Khabarova A.A., Yakovleva Yu.S., Lopatkina M.E., Kizilova E.A., Vasilyev S.A., Serov O.L., Lebedev I.N. Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22 // Stem Cell Research. 2018. P. 244-248. doi: 10.1016/j.scr.2018.08.012.